anti pten (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti pten
Anti Pten, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 684 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+pten/PTEN+(D4%2E3)+XP+Rabbit+mAb/10__36721_slash_pjps__2026__39__6__167__1-83-13-14
Average 96 stars, based on 684 article reviews

anti pten - by Bioz Stars, 2026-09

96/100 stars

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Western Blot:

Article Title: Selective anti-cancer effects of cannabidiol and Δ9-tetrahydrocannabinol via PI3K/AKT/mTOR inhibition and PTEN restoration in ovarian cancer cells
Article Snippet: .. Antibodies used for Western blotting were as follows: rabbit anti-mTOR (Cell Signaling Technology, Danvers, MA, United States, Cat# 2983), rabbit anti-Akt (Cell Signaling Technology, Cat# 4691), rabbit anti-PTEN (Cell Signaling Technology, Cat# 9559), rabbit anti-phospho-Akt (Cell Signaling Technology, Cat# 4060), and rabbit anti-GAPDH (Cell Signaling Technology, Cat# 5174), rabbit anti-PIK3CA (Thermo Fisher Scientific, Waltham, MA, United States, Cat# PA585091 ), rabbit anti-phospho-PI3K p85 alpha (Tyr607) (Thermo Fisher Scientific, Cat# PA5104853), rabbit anti-phospho-mTOR (Ser2448) (Thermo Fisher Scientific, Cat# MA533138 ), and rabbit anti-phospho-PTEN (S380/T382/T383) (ABclonal Technology, Wuhan, China, Cat# AP1346). .. Two thousand cells were seeded into 96-well culture plates (Corning, United States) for 12–16 h. After incubation, the medium was removed and replaced with different concentrations of CBD, THC, and combination in culture medium.

Article Title: Remote Control of Cell Signaling through Caveolae Mechanics
Article Snippet: .. The following primary antibodies were used: mouse anti-a-tubulin (Sigma-Aldrich, clone B512, Cat. #T5168, 1:1000 for WB); mouse anti-CHC (BD Transduction, Cat. #610500, 1:5000 for WB); mouse anti-STAT3 (Cell signaling, clone 124H6, Cat. #9139, 1:1000 for WB); rabbit anti-pSTAT3 (Tyr705) (Cell signaling technologies, clone D3A7, Cat. #9145, 1:1000 for WB, 1:100 for IF); rabbit anti-STAT1 (Cell signaling technologies, Cat. #9172, 1:1000 for WB); mouse anti-pSTAT1 (Tyr701) (Cell signaling technologies, clone 58D6, Cat. #9167, 1:1000 for WB, 1:100 for IF); mouse anti-Cav1 (BD Transduction, Cat. #610407, 1:1000 for WB); rabbit anti-Cav1 (Cell Signaling Technologies Cat. #3238S, 1:1000 for WB, 2-5 μg/condition for IP, 1:50 for dSTORM, 1:150 for IF); mouse anti-PTRF (BD Transduction Cat. #611258, 1:1000 for WB); rabbit anti-PTRF (Cat. #ab48824, Abcam – discontinued, 1:1000 for WB, 1:50 for dSTORM, 1:150 for IF); rabbit anti-JAK1 (Cell signaling technologies, Cat. #3332S, 1:2000 for WB); mouse anti-JAK1 (Santa Cruz Biotechnology, Cat. #sc-1677, 1:50 for STORM); rabbit anti-eNOS (Cell signaling technologies Cat. #32027S, 1:2000 for WB); rabbit anti-PTEN (Cell signaling technologies Cat. #9188S, 1:2000 for WB); rabbit anti-AKT (Cell signaling technologies Cat. #9272S, 1:2000 for WB); rabbit anti-pAKT (Ser473) (Cell signaling technologies Cat. #4060S, 1:2000 for WB); rabbit anti-PTP1B (Abcam Cat. #ab244207, 1:2000 for WB); rabbit anti-β1-integrin (Proteintech Cat. #12594-1-AP); rabbit anti-mCherry (Institut Curie Recombinant antibody platform, A-P-R #13). .. The following secondary antibodies were used: Donkey anti-mouse-HRP (Jackson ImmunoResearch, cat. #715-035-151) and Donkey anti-rabbit-HRP (Jackson ImmunoResearch, cat. #711-035-152) were used at a dilution of 1:5,000 for WB; Donkey anti-mouse-AF647 (Jackson ImmunoResearch, cat. #715-606-150) and Donkey anti-rabbit-CF680 (Biotium, cat. #20820) were used at a dilution of 1:200 and 1:400 respectively, for STORM imaging.

other:

Article Title: Repurposing drugs for treating the Neurobehavioral manifestations of PTEN hamartoma tumour syndrome
Article Snippet: The following primary antibodies were used: mouse anti-783 Neuron-specific beta-III Tubulin (TUJ1), R&D Systems (#MAP1195), 1:5000; chicken anti-MAP2, Aves 784 Labs (#MAP717984), 1:150; rabbit anti-phospho-Akt (Ser473), Cell Signalling (#4060), 1:200; rabbit 785 anti-phospho-4E-BP1 (Thr37/46), Cell Signalling (#2855), 1:200; rabbit anti-phospho-S6RP 786 (S235/S236), R&D Systems (#AF3918), 1:10000; rabbit anti-PTEN (138G6), Cell Signalling (#9559), 787 1:250.

Recombinant:

Incubation:

Article Title: Enhancing neural stem cell integration in the injured spinal cord through targeted PTEN modulation
Article Snippet: Proteins (20 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Cat# 1658004) on 4%–20% SDS gels and transferred to PVDF membrane (Merck, Cat# IPFL00010). .. Subsequently, membranes were blocked with non-fat skimmed milk and incubated with rabbit anti-PTEN (1:2000, Cell Signaling, Danvers, MA, USA, Cat# 9559), rabbit anti-phosphorylated 40S ribosome protein S6 (1:2000, Cell Signaling, Cat# 4856) for overnight at 4°C. .. The next day, membranes were washed three times with TBST and incubated with goat anti-rabbit IgG HRP-conjugated secondary antibody (1:5000, Cell Signaling, Cat# 7074) for 1 hour at room temperature, followed by detection with a chemiluminescent substrate (WESTSAVE Gold, Ab Frontier, Seoul, Korea).

Article Title: Melatonin Exerts Chondroprotective Effects Against Osteoarthritis by Promoting PI3K/AKT/FoxO3-Mediated Mitophagy.
Article Snippet: .. Membranes were blocked in 5% skim milk at room temperature for 1 h and incubated overnight at 4°C with primary antibodies against: rabbit anti- PTEN- induced putative kinase 1 (PINK1) (1:1000, ab216144, Abcam), Parkin (1:2000, PA5- 13399, Invitrogen, Carlsbad, CA, USA), LC3B (1:2000, ab192890, Abcam), p62 (1:10000, ab109012, Abcam), PI3K (1:1000, #4249, CST), phosphorylated (p)PI3K (1:1000, #4228, CST), p- AKT (1:3000, 28731–1- AP, Proteintech, Wuhan, Hubei, China), AKT (1:5000, 10176–2- AP, Proteintech), FoxO3 (1:5000, 10849–1- AP, Proteintech), COL2A1 (ab307674, 1:1000, abcam), MMP3 (ab52915, 1:5000, abcam), MMP13 (ab39012, 1:3000, abcam), and ADAMTS4 (ab314856, 1:1000, abcam). .. After washing, membranes were incubated with horseradish peroxidase- labeled goat anti- rabbit immunoglobulin G (1:5000, ab205714, Abcam) for 1 h at room temperature.

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